TABLE 3.
Septal recruitment of TolAIII by TolB, Pal, and CpoB
| Row | Hosta |
Plasmid |
Accumulation at septab | |||
|---|---|---|---|---|---|---|
| Name | Genotype | Still presentc | Name | Genotype | ||
| 1 | TB28 | wt | tolQRAB, pal, cpoB | + | ||
| 2 | LP49 | ΔtolA | tolQRB, pal, cpoB | + | ||
| 3 | LP56 | Δ(tolQ-tolA) | tolB, pal, cpoB | + | ||
| 4 | LP50 | Δ(tolA-pal) | tolQR, cpoB | − | ||
| 5 | BL78 | ΔcpoB | tolQRAB, pal | − | ||
| 6 | LP57 | Δ(tolQ-cpoB) | − | |||
| 7 | LP57 | Δ(tolQ-cpoB) | pBAD33 | PBAD:: | − | |
| 8 | LP57 | Δ(tolQ-cpoB) | pMG45 | PBAD::tolB | − | |
| 9 | LP57 | Δ(tolQ-cpoB) | pCH546 | PBAD::cpoB | − | |
| 10 | LP57 | Δ(tolQ-cpoB) | pCH518 | PBAD::tolB pal | − | |
| 11 | LP57 | Δ(tolQ-cpoB) | pCH545 | PBAD::pal cpoB | − | |
| 12 | LP57 | Δ(tolQ-cpoB) | pCH528 | PBAD::tolB pal cpoB | + | |
| 13 | LP57 | Δ(tolQ-cpoB) | pCH544 | PBAD::tolB pal0 cpoB | − | |
| 14 | LP57 | Δ(tolQ-cpoB) | pCH548 | PBAD::tolB pal cpoB0 | − | |
| 15 | LP49 | ΔtolA | tolQRB, pal, cpoB | − | ||
| 16 | LP57 | Δ(tolQ-cpoB) | pCH528 | PBAD::tolB pal cpoB | − | |
a
Strains lysogenic for λCH536 (Plac::gfp-malF2-39-rodZ139-255-tolA294-421) (rows 1 to 14) or λCH543 (Plac::gfp-malF2-39-rodZ139-255) (rows 15 and 16) and transformed with the indicated plasmid (rows 7 to 14 and 16) were grown for ∼3.5 mass doublings to OD600 = 0.5 to 0.6 in M9-maltose medium with 37 μM IPTG and either no (all rows) or 0.01% (rows 7 to 14 and 16) arabinose. Note that the results in rows 7 to 14, and 16 were the same with or without the addition of arabinose. See also Table S2.
b
GFP fluorescence distributed evenly along the periphery of cells (−) or accumulated at sites of cell constriction (+).
c
Genes of the tolQ-cpoB cluster that are still present on the chromosome.