TABLE 4.
Functional fluorescent ZipA sandwich fusions
| Straina | Genotype | Result in LBb |
Result in M9-glucose mediumc |
||||
|---|---|---|---|---|---|---|---|
| Lengthd | Widthd | n e | Lengthd | Widthd | n e | ||
| TB28 | wt | 4.18 (0.88) | 0.90 (0.07) | 838 | 2.43 (0.50) | 0.96 (0.08) | 2,027 |
| CH119 | TB28, yfeN<>aph | 4.24 (0.89) | 0.90 (0.07) | 609 | 2.53 (0.53) | 0.97 (0.09) | 1,764 |
| CH125 | CH119, zipA-rfpSW | 4.14 (1.00) | 0.87 (0.09) | 1622 | 2.74 (0.58) | 0.97 (0.08) | 2,177 |
| CH128 | CH119, zipA-SFgfpSW | 4.18 (0.97) | 0.96 (0.08) | 1480 | 2.61 (0.57) | 0.98 (0.09) | 2,158 |
a
Cells were chemically fixed, imaged with phase contrast optics, and analyzed using MicrobeJ software (115).
b
Cells were grown for ∼5 mass doublings to OD600 = 0.65 to 0.70 before fixation.
c
Cells were grown for ∼3.5 mass doublings to OD600 = 0.55 to 0.60 before fixation.
d
Mean in micrometers, with standard deviation in parentheses.
e
Number of cells measured.