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. 2022 Jan 14;15:311–324. doi: 10.2147/JIR.S342399

Figure 1.

Figure 1

Dietary fish oil increases the number of CD11b+CD27− NK cells in peritoneum of mice 6 h after inflammation induction. Mice were fed control (Ctr, grey line with grey circles) or fish oil (Fo, black line with black squares) diets for 5 weeks. They were immunized twice with mBSA with a 2-week interval and subsequently challenged intraperitoneally. Mice were sacrificed at 0, 1.5, 3, and 6 h following challenge and peritoneal cells collected. Peritoneal cells were counted with a Countess automated cell counter, stained with monoclonal antibodies against CD3, NK1.1, CD49b (DX5), CD11b and CD27 and evaluated by flow cytometry. Representative gating strategy on CD3− lymphocytes to identify NK cells based on their NK1.1 and CD49b (DX5) expression (A) and separation of NK cells into 4 distinct subtypes based on their CD27 and CD11b expression (B) 6 h after inflammation induction in mice fed either Ctr or Fo diets. Number of CD3−NK1.1+CD49b (DX5)+ lymphocytes at different timepoints following induction of inflammation (C) and of those the number of CD11b−CD27−, CD11b−CD27+, CD11b+CD27+, and CD11b+CD27− cells at 6 h after inflammation induction (D). *p < 0.05, ***p < 0.001. n = 9–12 for the 0 and 3 h time-points and 35–48 for 6 h post-challenge. Results are shown as mean ± standard error of the mean from data collected from at least four independent experiments.