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. 2022 Jan 14;15:311–324. doi: 10.2147/JIR.S342399

Figure 2.

Figure 2

Dietary fish oil increases the number of CCR5+ NK cells and enhances CCL5 and CXCL12 concentrations in peritoneum 6 h after induction of inflammation. Mice were fed control (Ctr, grey line with grey circles) or fish oil (Fo, black line with black squares) diets for 5 weeks. They were immunized twice with mBSA with a 2-week interval and subsequently challenged intraperitoneally. Mice were sacrificed at 0, 1.5, 3, and 6 h following challenge and peritoneal lavage collected. Peritoneal cells were counted with a Countess automated cell counter, stained with monoclonal antibodies against CD3, NK1.1, CD49b (DX5), and CCR5 and analyzed by flow cytometry. Representative dot plots of NK cell expression of CCR5 in mice receiving Ctr and Fo diets 6 h after inflammation induction (A). Number of CCR5+ NK cells at 0, 1.5, 3, and 6 h (B) and peritoneal concentrations of CCL5 and CXCL12 6 h (C) following inflammation induction in mice receiving Ctr and Fo diets. ***p < 0.001, n = 8–15 (B) and n = 47–50 (C). Results are shown as mean ± standard error of the mean from data collected from at least four independent experiments.