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. 2022 Jan 4;15:720286. doi: 10.3389/fnins.2021.720286

FIGURE 6.

FIGURE 6

Maternal separation induces the expression of different glutamate levels in rat PrL in adolescence and adulthood. (A) The experimental timeline of MS, microdialysis and whole-cell recording (PPR analysis). (B) Schematic diagram of microdialysis experiment flow. (C) A coronal view showing the microdialysis probe at prelimbic cortex. (D,E) Represents the changes of glutamate and GABA level in mPFC recorded by microdialysis in vivo in adolescent rats (n = 6–7). (F,G) Represents the changes of glutamate and GABA level in mPFC recorded by microdialysis in vivo in adult rats (n = 5–6). (H) PPR evoked by paired stimuli (interstimulus interval: 50, 100, 150, and 200 ms) in mPFC L2/3-L5 synapses for control and MS in adolescent (n = 15 cells, 3–4 rats each group). Right: Representative traces PPR with interstimulus interval of 50 ms for control and MS in adolescent. Scale bars: 400 pA, 40 ms. (I) PPR evoked by paired stimuli (interstimulus interval: 50, 100, 150, and 200 ms) in mPFC L2/3- L5 synapses for control and MS in adult (control: n = 3–4 rats, cell = 15; MS: n = 3–4 rats, cell = 20). Right: Representative traces PPR with interstimulus interval of 50 ms for control and MS in adolescent. Scale bars: 400 pA, 40 ms. *p < 0.05, **p < 0.01. Data are the means + SEM.