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Effect of IDH suppression on epigenetic marks in JB6 cells. (A) Suppression of IDH2/3 by treating JB6 cells with respective siRNA. Proteins were then detected by western blot analysis using antibodies and quantified in (B). (C-D) Activity of isocitrate dehydrogenase following suppression of IDH2/3 using siRNA. Data points are presented as the mean +/− SD of three individual samples. Statistical significance is indicated by asterisks: **, p ≤ 0.01.
