Fig 5. ADCP and complement binding activity of V3 mAb 2219 with WT or mutated Fc fragments (LALA and KA).

A) ADCP activity was measured using gp120 ZM109-coated beads and THP-1 phagocytic cells in the presence of 2219 WT or Fc mutants. Statistical test was done using two-way ANOVA. B) Complement binding by V3 mAb 2219 with WT and mutated Fc fragments was detected in ELISA or multiplex bead experiments in which C1q or C3d deposition to mAbs in complex with V3 MN peptide was measured with anti-C1q or anti-C3d secondary antibodies. Comparison was analyzed with two-way ANOVA. C) ADCP of 2219 WT and Fc mutants was detected by measuring phagocytosis of mAb-treated gp120 ZM109-coated beads by resident peritoneal macrophages from NSG mice.