Fig 6. Detection and quantification of the cytokines TNF-α and IL-10 in fatal cases of DENV.

(A) Detection of IL-10 in focal areas and few macrophages in both fatal cases. Control tissues exhibited a high expression of IL-10. (B) Quantitative analysis of IL-10 showed a significantly decrease in fatal cases compared to controls. (C) Detection of TNF-α in macrophages of pancreatic islets and underlying connective tissue. TNF-α was not detected in control tissues. (D) Quantitative analysis of TNF-α revealing an increase in the expression of this cytokine compared to controls. Data are represented as mean ± SDM. (a and b) indicate differences that are statistically significant between individual specimens (p < 0.05).