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. 2022 Jan 4;12:771216. doi: 10.3389/fimmu.2021.771216

Figure 2.

Figure 2

miR-23b-3p has a positive effect on EMT. (A) RT-qPCR analysis of mRNA levels of miR-23b-3p after transfection of miR-23b-3p mimic or miR-23b-3p inhibitor in BEAS-2B cells. (B) RT-qPCR analysis of mRNA levels of EMT markers E-cadherin, N-cadherin, and vimentin after transfection of miR-23b-3p mimic or miR-23b-3p inhibitor in BEAS-2B cells with or without TGF-β1 treatment. (C) Western blot analysis of protein levels of EMT markers E-cadherin, N-cadherin, and vimentin after transfection of miR-23b-3p mimic or miR-23b-3p inhibitor in BEAS-2B cells with or without TGF-β1 treatment. (D) Representative images and quantitation of Transwell assay after transfection of miR-23b-3p mimic or miR-23b-3p inhibitor in BEAS-2B cells with or without TGF-β1 treatment. Representative photomicrographs (magnification 100×) are demonstrated for each subject. The results are presented as the means ± SD of three independent experiments. *P < 0.05, ***P < 0.001 compared with the indicated groups. #P < 0.05 compared with the indicated groups.