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. Author manuscript; available in PMC: 2022 Jun 10.
Published in final edited form as: Sci Immunol. 2021 Dec 10;6(66):eabj0789. doi: 10.1126/sciimmunol.abj0789

Fig. 2: Role of TCF-1 in enhanced proliferation of infant T cells.

Fig. 2:

(A) Expression of TCF-1 as a function of proliferation (CPD expression) at day 4 post infection in medLN of host mice shown in flow cytometry plots (left) and graphs of paired frequencies of CPDlow TCFlow infant and adult OT-II cells (right). (B) TCF-1 expression shown in flow cytometry plots (top) and graphs (bottom) of individual paired frequencies of TCF-1+ infant (red) and adult (blue) lung effector cells. (C) Left: Representative flow cytometry plots of transferred infant OT-II/TCF-1 Tg CD45.1− (red) and adult CD45.1+ (blue) OT-II cells in LNs of host mice at day 0. Middle: Paired frequencies of infant and adult OT-II cells within individual host mice. Right: Baseline expression of TCF-1 in OT-II/TCF-1 Tg infant (red) compared to adult (blue) cells in LNs at day 0. (D) Total number of OT-II/TCF-1 Tg infant (red) and OT-II adult (blue) cells in lung (left) and medLN (right) of individual mice at indicated days p.i.(E) Ratio of infant OT-II/TCF-1 Tg cells:adult OT-II cell numbers in lung and medLN of congenic hosts post infection. (F) Ratio of OT-II in lung:medLN from OT-II adult or OT-II/ TCF-1 Tg infant in congenic hosts post infection derived from cell numbers. Data are representative of 2 independent experiments with n=3-4 mice per experiment. Statistical analysis was done using Student’s paired t-test (*p<0.05) with error bars representing S.E.M.