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. Author manuscript; available in PMC: 2022 Jun 10.
Published in final edited form as: Sci Immunol. 2021 Dec 10;6(66):eabj0789. doi: 10.1126/sciimmunol.abj0789

Fig. 6: Infant T cells have higher accumulation of signaling molecules in the immunological synapse compared to adult T cells.

Fig. 6:

(A) Schematic diagram of ImageStream analysis using IDEAS software to examine accumulation of actin, Lck, and CD4 in the immune synapse of conjugates upon stimulation (see ImageStream Analysis section in Materials and Methods). (B) Infant T cells compared to adult T cells have higher accumulation of actin (red) with CD3 (turquoise) in T cell:APC conjugates as shown in representative images. OT-II cells were stimulated with 10 μg peptide pulsed APC for indicated times and fixed immediately before staining. (C) Infant T cells compared to adult T cells have higher accumulation of Lck (yellow) with CD3 (turquoise) in T cell:APC conjugates as shown in representative images. (D) Infant T cells compared to adult T cells have higher accumulation of CD4 (pink) with CD3 (turquoise) in T cell:APC conjugates as shown in representative images. (E-G) Graphs quantifying fold change (FC) of actin (E), Lck (F), and CD4 (G) relative to unstimulated control (right). Data are from 3 independent experiments. Significance was determined using two-way ANOVA with Sidak multiple comparison testing.