Figure 3. Hematopoietic domains in 14 hpf surface fish and cavefish embryos.
(A). In situ hybridizations showing expression of the gfi1aa (top frames) and hbb2 (bottom frames) genes in the anterior lateral mesoderm (ALM, arrowheads) and posterior lateral mesoderm (PLM, arrows) in surface fish (SF) and cavefish (CF). (B) Expression of the macrophage marker gene lcp1 in SF and CF embryos. (C). Expression of the hematopoietic marker gene lmo2 in the ALM and PLM of CF and SF embryos, and expansion of expression in CF embryos. Scale bar in A is 200 µm; magnification is the same in all frames.