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. 2022 Jan 5;18(1):e1009993. doi: 10.1371/journal.pgen.1009993

Fig 6. Overexpression of the dominant-negative FtsW mutants does not prevent localization of GFP-FtsI.

Fig 6

Overnight cultures of LYA4 (TB28, zapA-mcherry cat<>frt) carrying plasmid pLY114 (pBAD33, PBAD::gfp-linker-ftsI) were diluted 1:100 in fresh LB medium with antibiotics and cultured at 30°C. 2 hours later the cultures were diluted 1:20, IPTG was added to a final centration of 250 μM to induce the expression of the FtsW mutants and arabinose was added to a final concentration of 0.05% to induce expression of GFP-FtsI. 3 hours post induction cells were immobilized on 2% agarose pads for photographing. Scale bar, 3 μm.