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. 2022 Jan 5;18(1):e1009993. doi: 10.1371/journal.pgen.1009993

Fig 9. Purification of FtsW variants in a complex with FtsI/PBP3.

Fig 9

FtsW and FtsI/PBP3 were co-expressed in E. coli cells; FtsW contains a His-Tag and was used as bait to co-purify untagged FtsI/PBP3 on a HisTrap column. The samples were incubated with Bocillin FL to label FtsI and analyzed by SDS-PAGE. The gels were first subjected to fluorescence imaging to detect Bocillin-bound FtsI/PBP3 (B) followed by Coomassie blue staining (A). The positions of FtsW and FtsI/PBP3 are indicated by arrows and FtsW mutations are shown on top of each lane of the gel. FtsW* indicates an FtsW degradation product. M, protein standards.