Table 1. Dominant-negative FtsW mutations isolated from the wrinkle-colony-based screen.
| Mutation | # of isolates | Dominant-negative effect | Complementation | Location of the mutation |
|---|---|---|---|---|
| K133I | 1 | ++ | - | ECL2 |
| A135T | 2 | ++++ | - | ECL2 |
| A135V | 1 | ++++ | - | ECL2 |
| R137H | 1 | ++ | - | ECL2 |
| Q147E | 1 | ++++ | - | ECL2 |
| Q147K | 1 | ++ | - | ECL2 |
| P196T | 1 | ++ | - | ECL3 |
| L198I | 1 | + | - | ECL3 |
| G199A | 2 | +++ | - | ECL3 |
| Y242H | 1 | ++++ | - | ECL4 |
| R243H | 1 | +++ | - | ECL4 |
| R243L | 1 | ++++ | - | ECL4 |
| R243P | 1 | ++ | - | ECL4 |
| R243S | 1 | +++ | - | ECL4 |
| R246H | 2 | ++ | - | ECL4 |
| G371S | 1 | +++ | - | ECL5 |
| G381V | 2 | ++ | - | ECL5 |
| G381D | 1 | ++ | - | ECL5 |
Dominant-negative effect: “++++”, “+++”, “++” and “+” indicate that it takes ≤60 μM, 60–125 μM, ≥125 μM or ≥1000 μM IPTG to induce the expression of the mutant to completely block colony formation, respectively. Complementation: “-” indicates that the mutant cannot complement an ftsW depletion strain.