Table 2. Amino acid substitutions in FtsW generated by site-directed mutagenesis.
| Mutation | Dominant-negative effect | Complementation | Location of the mutation |
|---|---|---|---|
| W138A | ++++ | - | ECL2 |
| E150A | - | + | ECL2 |
| H295A | - | + | ECL4 |
| T296A | - | + | ECL4 |
| D297A | ++ | - | ECL4 |
| P368A | - | + | ECL5 |
| S378A | - | + | ECL5 |
| Y379A | ++ | - | ECL5 |
| G380A | ++ | - | ECL5 |
Dominant-negative effect: “++++” and “++” indicate that it takes ≤60 μM or 60~125 μM IPTG to induce the expression of the mutant to completely block colony formation, respectively. “-” indicates the mutants do not display a dominant-negative effect. Complementation: “+” and “-” indicate that the mutant can or cannot complement an ftsW depletion strain, respectively.