Figure 2.

Overexpression of GAS5-sensitized cancer cells to IR. (a) Relative expression of GAS5 was detected by real-time PCR in the vector- and lncRNA GAS5-overexpressing MCF-7 cells at 48 h after transfection. (b) The viability of MCF-7 cells was determined with a CCK-8 kit at different times (0, 24, and 48 h) postirradiation (8 Gy). (c) Representative images of cell apoptosis were obtained with flow cytometry of MCF-7 cells with/without GAS5 and radiation treatments. (d) Quantitative analysis of cell apoptosis (upper right quadrant plus lower right quadrant) after different treatments. ^∗P < 0.05 and ^∗∗P < 0.01 vs. the control group.