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Overexpression of GAS5 resulted in increased G2/M cell cycle arrest and DNA damage. (a) The cell cycle distribution was analyzed by flow cytometric analysis of MCF-7 cells at 12 h after 8 Gy irradiation. (b) Representative images of the comet assay of the MCF-7 cells with 8 Gy irradiation and/or GAS5 overexpression. (c) Bar graph of the tail moment of the comet assay analyzed with CASP software in the cells from different groups. ∗P < 0.05 and ∗∗P < 0.01 vs. the control group.
