Skip to main content
. 2022 Jan 11;2022:1987519. doi: 10.1155/2022/1987519

Figure 4.

Figure 4

GAS5 regulated radiosensitivity by interacting with miR-21. (a) Relative expression level of miR-21 in the vector- and GAS5-overexpressing MCF-7 cells. (b, c) Analysis of cell viability and apoptosis of the MCF-7 irradiated cells (8 Gy) transfected with GAS5 and/or miR-21 mimics. For analysis of cell viability, CCK-8 assays were performed at 48 h. ∗P < 0.05 and ∗∗P < 0.01 vs. the GAS5-overexpressing group. (d) Expression of miR-21 in breast cancer cells was detected with real-time PCR assay. ∗P < 0.05 vs. that in MCF10A cells. (e) Sequence of wild-type and mutant GAS5 and seed sequence of miR-21 binding site. (f) Luciferase assay of lncRNA GAS5 and miR-21 cotransfected cells. ∗P < 0.05 vs. the GAS5 expression cells. ns means the comparison between miR-21 plus mutant GAS5 expression cells.