FIG. 1.

(A) Specific amplification of BoHV-4 DNA fragment from PBMC samples from BoHV-4 IFAT-positive cows. PCR amplified a 2,538-bp fragment of the BoHV-4 genome containing ORF1 overlapping ORF2 gene, ORF3 corresponding to the thymidine kinase gene, and ORF4 homologous to the herpes simplex virus type 1 glycoprotein H gene. Lanes 1 to 7 correspond to PBMC samples; lanes − and + correspond to negative (DNA from PBMC of BoHV-4 seronegative cow) and positive controls, respectively. 1 kb, molecular size marker. (B) Predicted location of HindIII restriction sites and respective expected restriction fragment sizes (not shown to scale). (C) Ethidium bromide-stained gel of HindIII-digested 2,538-bp BoHV-4 amplified sequence from PBMC DNA samples (lanes 1 to 7). 1 kb, molecular size marker.