Table 3.

PCR-based methods registered in the Genetic Testing Registry (GTR) for biomarker analysis regarding drug response

PCR-based methods ‘Molecular genetics’	Percentage of drugs (‘Testing recommended’ and ‘Testing required’) (%)	Percentage of drugs (‘Actionable PGx’) (%)
ARMS	3.4	–
ASPE	19.0	53.1
KASP	5.2	43.8
MLPA	43.1	62.5
PCR	36.2	62.5
QF PCR	–	6.3
qPCR	29.3	56.3
RFLP	12.1	50.0
RT-PCR/ RT-qPCR	50.0	56.3

Most frequenty applied techniques in both listings (‘Testing recommended’/‘Testing required’ and ‘Actionable PGx’) are marked in bold

ARMS amplification refractory mutation system, ASPE allele-specific primer extension, KASP competitive allele-specific PCR, MLPA multiplex ligation-dependent probe amplification, PCR polymerase chain reaction, QF-PCR quantitative fluorescent PCR, qPCR quantitative real-time PCR, RT-PCR reverse transcriptase PCR, RFLP Restriction Fragment Length Polymorphism Analysis