Table 7.

Overview of selected sequencing applications

Sequencing technique	Mechanism [46]	Maximal reads length	Advantages	Disadvantages
Illumina Sequencing	Sequencing by synthesis (cyclic reversible termination)	2 × 150-300 bp [56]	High throughput sequencing [75]	Short read length [46]
Ion Torrent™ semiconductor sequencing	Sequencing by synthesis (single-nucleotide addition)	200-600 bp [76]	Fast turnaround time, flexibility [77]	Accuracy and output affected by read length [78, 79]
SMRT from Pacific Bioscience	Single-molecule real-time long read sequencing	up to 25 kb [80]	Long read length [80]	High error rate [81]
Oxford Nanopore’s sequencing	Single-molecule real-time long read sequencing	> 4 Mbp [82]	Ultra-long reads [82]	High error rate [83]

bp base pairs, kbp kilo base pairs, Mbp mega base pairs