Table 1.
Antibodies used for flow cytometry, NK cell depletion, and immunofluorescence staining
| Antigen | Reactivity | Supplier | Order No | |
|---|---|---|---|---|
| Flow cytometry | ||||
| CD3 | Mouse | BioLegend | 100,218 | |
| CD4 | Mouse | BioLegend | 100,531 | |
| CD8a | Mouse | BioLegend | 100,730 | |
| CD11b | Mouse/Human | BioLegend | 101,263 | |
| CD11c | Mouse | BioLegend | 117,306 | |
| CD43 | Mouse | BioLegend | 143,205 | |
| CD45 | Mouse | BioLegend | 103,116 | |
| CD27 | Mouse | BioLegend | 124,215 | |
| CD45R(B220) | Mouse | BioLegend | 103,208 | |
| CD49b | Mouse | BioLegend | 108,915 | |
| CD62L | Mouse | BioLegend | 104,418 | |
| CD122 | Mouse | Beckman Coulter | 732,491 | |
| CD107a | Mouse | BioLegend | 121,616 | |
| DNAM-1 | Mouse | BD pharmingen | 565,549 | |
| KLRG1 | Mouse/human | BioLegend | 138,415 | |
| Ly49A | Mouse | BioLegend | 138,703 | |
| Ly49D | Mouse | BioLegend | 103,708 | |
| Ly49H | Mouse | Miltenyi | 130–5321-80 | |
| NK1.1 | Mouse | BioLegend | 108,710 | |
| NKG2D | Mouse | eBioscience | 12–5882-81 | |
| Nkp46 | Mouse | Miltenyi | 130–102-300 | |
| NK cell depletion | ||||
| NK1.1 (clone PK136) | Mouse, human | Bio X Cell | BE0036 | |
| Immunofluorescence staining | ||||
| NKp46 | Mouse, human | Abcam | ab233558 | |
| NeuN | Mouse | Millipore | MAB377 | |