Fig. 5. Break point of efficiency 80% (BP80).

Soraphen A (1), C (2), and enzymatically derivatized analogs (1a, 1b, 1c, 2a) were tested against six different fungi, determined by dilution series at four concentrations and measured against positive and negative standards. BP80 represents the concentration of active ingredients above which 80% or more of efficiency is observed. The experiments on living organisms were performed in two test series (biological replicates). The first series consisted of a single experiment (N = 1, square) for each fungus, the second series consisted of triplicates with three samples tested in parallel during the same test session (N = 3, triangles).