Table 1.

Biochemical characterization of selected WelO5* variants for the biocatalytic production of 1a.

Variant	app. kcat (min−1)	app. Km (mM)	app. kcat/Km (min−1 mM−1)	rel. kcat	TTN+
GAP	0.026 ± 0.007	0.45 ± 0.14	0.07 ± 0.21	1	0.3 ± 0.2
SLP	2.413 ± 0.349	0.42 ± 0.09	5.74 ± 0.07	93	30.0 ± 8.3
VLA	1.959 ± 0.509	0.44 ± 0.03	4.45 ± 0.07	75	91.8 ± 22.0

⁺(TTN experiments were performed in two test series (biological replicates) and each series consisted of four independent experiments (N = 4); kinetic parameters are given as the average of N = 3 ± SD).