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DSF analysis of stability of Shank3 constructs (1–348 WT and 1–348 P141A) in 0.5 M NaCl, 20 mM Tris, 2 mM DTT, pH 7.5. Fluorescence based on SYPRO-Orange dye. Mean normalised data plotted showing melt curves (a) and Tm values calculated from V50 Boltzman calculation from mean fluorescence data (b); ****Significantly different from WT, p < 0.0001; data from three independent experiments; t-Test.
