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. 2022 Jan 18;8:11. doi: 10.1038/s41523-021-00372-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Representative images of Boyden chamber migration assay of BT-20 wt and two cellular clones of CEBPB-ko cells with at the right a bar graph of corresponding quantifications (wt, n = 3; CEBP-ko n = 4). One-way ANOVA analysis; error bars represent SD, *p < 0.05, **p < 0.01, ***p < 0. 001, ****p < 0.0001. scale bar represents 100 μm. b Bar graph with representative quantification of 3D Boyden chamber-matrigel invasion assay using BT-20 wt and BT-20 CEBPB-ko clone #1 (BT-20 wt, n = 6; and BT-20 CEBPB-ko, n = 6). Statistical differences were analysed after 48 h using an unpaired T-test. Error bars represent SD, *p < 0.05, **p < 0.01, ***p < 0. 001, ****p < 0.0001. c Immunoblot showing the expression of LAP and LIP in BT-20 cells containing a cumate-inducible LAP construct or empty vector control (EV), two cellular clones of each. β-actin was used for loading control. d Time course of Incucyte scratch wound migration assay shown as relative wound density of BT-20 cells containing a cumate-inducible LAP construct (clone #1) or empty vector control (EV, clone #1) induced with cumate (n = 4). Statistical differences were analysed for timepoints 24 and 48 h using Two-way Anova analysis and Tukey’s multiple comparisons test (BT-20 EV− cumate vs BT-20 EV+ cumate were compared (ns), and BT-20 LAP− cumate vs BT-20 LAP+ cumate were compared (**)). Error bars represent SD, *p < 0.05, **p < 0.01, ***p < 0. 001. e Bar graph shows quantification of 3D Boyden chamber-matrigel invasion assay using BT-20 cells containing a cumate-inducible LAP construct (clone #1) or empty vector control (EV, clone #1) (BT-20 EV, n = 3; BT-20 LAP, n = 4). Statistical differences were analysed after 48 h using two-way Anova analysis. Error bars represent SD, *p < 0.05, **p < 0.01, ***p < 0.001. f Immunoblot shows the expression of LAP and LIP in wt MEFs and LIP-deficient Cebpb^ΔuORF MEFs with the respective LIP/LAP ratios shown underneath. α-tubulin was used for loading control. The bar graph shows relative migration rate of a scratch wound assay (n = 5). Statistical differences were analysed by T-test. Error bars represent SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. g Immunoblot shows the re-expression of LAP and LIP in Cebpb-ko MEFs as well as empty vector control (EV). β-actin was used for loading control. The bar graph shows relative migration rate of a scratch wound assay (n = 5). Statistical differences were analysed by T-test. T Error bars represent SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The immunoblots from (f) and (g) are taken from from Ackermann et al.²⁸.