Table 5.
Increase of purity fold corresponding to various affinity separation operations carried out and their effect on the yield and specific enzyme activity of fibrinolytic protease.
| Purification steps | Total protein (TP) mg/ml | Enzyme activity (EA) U/ml | Specific activity (SA) U/mg | Recovery (%) | Purification fold |
|---|---|---|---|---|---|
| Crude | 402.5 | 21,131.85 | 52.5 | 100 | 1 |
| Extractive fermentation | 94.2 | 18,670 | 198.1 | 88 | 3.77 |
| Gel filtration chromatography | 8.5 | 7217 | 849.05 | 34.1 | 16.1 |
| Ion exchange chromatography | 2.22 | 2603.2 | 1172 | 12.3 | 22.32 |
Relative uncertainty for the total protein, enzyme activity and specific activity are ur (TP) = 0.2 mg/ml, ur (EA) = 0.02 U/ml, ur (SA) = 0.02 U/mg.