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. 2022 Jan 19;414(10):3177–3186. doi: 10.1007/s00216-022-03880-4

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© The Author(s) 2022

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Fig. 2 — The results of preliminary tests for confirmation of LAMP and LFA compatibility. A Colorimetric LAMP with SYBR-Green produced a bright green color in (a) the positive sample and dull orange color in (b) the non-template control. B LAMP products were observed to show a “ladder like” band pattern when the amplification was successful. Lanes: (a) marker (100 bp), (b) non-template control 1, (c) cDNA LAMP-amplified product with 10⁹ copies/μL, (d) repetitive sample for LAMP-amplified cDNA with 10⁹ copies/μL, (e) marker (100 bp), (f) non-template control 2, (g) cDNA LAMP-amplified product with 10⁴ copies/μL, (h) repetitive sample for LAMP-amplified cDNA with 10⁴ copies/μL, and (i) marker (100 bp). In C, the LAMP product was read out on the test strip. Test and control lines appeared as expected—positive readout: both lines, negative readout: only control line. Stripes/samples: (a) negative readout for the non-template control, (b) positive readout for cDNA with biotin-LB primer and FITC-LF primer, (c) positive readout for cDNA with biotin-dUTP (5%) and FITC-LF primer, and (d) negative readout for the non-template control