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. 2022 Jan 19;414(10):3177–3186. doi: 10.1007/s00216-022-03880-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — LAMP-LFA experiments with cDNA. A LFA readout for (a) to (j): cDNA LAMP-amplified product, showing a clear control line (CL) and test line (TL); (k) to (t): non-template controls for each of the cDNA LAMP products, showing only control lines (CL). B The contingency table for all the cDNA LAMP experiments, where a total of 161 samples were tested. C The sensitivity = 95.49%, specificity = 96.43%, and accuracy = 95.65% of the cDNA-LAMP-LFA results