Fig. 4.

RT-LAMP-LFA experiments with RNA extracted from clinical COVID-19-positive swab samples. A The RT-LAMP-LFA readout for (a) non-template control, (b) RNA with CT-33, (c) RNA with CT-29, (d) RNA with CT-26, (e) RNA with CT-22. The test lines are visible for the RNA-positive samples, while the NTC only showed the control line. Out of the SARS-CoV-2 variants tested with RT-LAMP-LFA, B the variants of concern alpha and delta were detected by our assay system similar to the wild-type strain. C The contingency table for the 169 RT-LAMP-LFA experiments. D The sensitivity = 77.27%, specificity = 97.30%, and accuracy = 81.66% of the clinical RNA RT-LAMP-LFA results