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. 2022 Jan 5;12:764861. doi: 10.3389/fimmu.2021.764861

Figure 6.

Figure 6

Inhibition of IRE-1α in HSV-infected DCs promotes the migration of DCs from the skin to draining lymph nodes and the activation of virus-specific T cells in vivo. (A) Migration of DCs treated with 4µ8c or MKC and then inoculated with HSV-1 or HSV-2, from the hindlimb footpads to draining popliteal lymph nodes (pLNs). Inoculated DCs were stained with CFSE and detected in the draining lymph node (CFSE+-gated, then CD11c+/MHC-II+/Zombie–-cells-analysed). (B) CD69 and CD71 surface expression in virus-specific gBT-I CD8+ T cells in pLNs 48 h after the inoculation of IRE-1α inhibitor-treated and HSV-1 or HSV-2-infected DCs in the footpads. (C) CD69 and CD71 surface expression in virus-specific gDT-II CD4+ T cells 48 h after the inoculation of IRE-1α inhibitor-treated and HSV-1 or HSV-2-infected DCs in the footpads. One-way ANOVA with Tukey’s multiple comparison test were used for statistical analyses (*p < 0.05, ***p < 0.001). Data are means ± SEM (n = 2 mice/group).