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. 2022 Jan 5;12:790415. doi: 10.3389/fimmu.2021.790415

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Copyright © 2022 Vanderheijden, Stevaert, Xie, Ren, Barbezange, Noppen, Desombere, Verhasselt, Geldhof, Vereecke, Stroobants, Oh, Vanhee, Naesens and Nauwynck

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reactivity of cat samples in seroneutralization assays and pepscan. Samples from FIP cats (cats 3, 4, 7, 19 and 22) were collected between 2004 and 2007, the other samples were collected in 2020 for diagnosis of FeCV (cats 164, 243 and 245) or SARS-CoV-2 (cat 40). The virus-neutralizing activity (left) was determined by seroneutralization (SN) assay against FeCV in feline enterocytes, and SARS-CoV-2 in VeroE6 cells. The limit of detection (LOD) was a 1:8 (FeCV) or 1:40 dilution (SARS-CoV-2). A pepscan (right) was performed using PEP1-4 (derived from SARS-CoV-2 S), PEP71 (HCoV-OC43 S) and PEP72 (HCoV-229E S). The fold increase in OD_{450 nm} value is shown versus negative control peptide PEP74.