Skip to main content
. 2022 Jan 18;42(1):BSR20212062. doi: 10.1042/BSR20212062

Figure 4. miR-24-3p directly targets NKAP in hypoxic HUVECs.

Figure 4

(A) The predicted binding of miR-24-3p with the NKAP 3′-UTR. (B) A dual-luciferase reporter assay was performed to confirm NKAP as the miR-24-3p target in hypoxic HUVECs. NKAP linked to the firefly luciferase reporter were transfected into hypoxic HUVECs. The Renilla luciferase reporter construct pRL-TK was also used in the co-transfection to monitor the transfection efficiency. Relative luciferase activity was firefly luciferase expression was normalized to Renilla luciferase. (C) Western blotting was performed to validate the NKAP protein levels in hypoxic HUVECs transfected with miR-24-3p or miR-NC. The protein expression level, quantified by band intensity and normalized to β-tubulin, is displayed in the right panel. All of the experiments were repeated at least three times. Data represent the mean ± SD; N.S., not significant, **P<0.01.