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Multiple sequence alignment based on amino acid sequences of BoLDH and related LDHs. BoLDH sequence were aligned with other protozoans and mammals, and the multiple sequence alignment were created by ClustalW. Black triangles indicated the key residues for NADH, and black solid circles indicated the key residues for substrate analog oxamate. The residues were labeled based on the holo PfLDH (PDB accession number 1t2d, Cameron et al., 2004).
