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Kinetic parameters of BoLDH on different substrates and cofactors.
| Parameter | Pyruvate | NADH | Lactate | NAD+ | APAD+ | Lactate (APAD+) |
|---|---|---|---|---|---|---|
| K M (μM) | 102 | 126 | 166 | 493 | 395 | 12.9 |
| V max (μM μg-1 min-1) | 458 | 929 | 247 | 174 | 502 | 351 |
| K cat (S-1) | 56.5 | 115 | 30.5 | 21.5 | 62.3 | 43.3 |
| K cat/K M (S-1 M-1) | 5.54 × 105 | 9.13 × 105 | 1.84 × 105 | 4.36 × 104 | 1.57 × 105 | 3.36 × 106 |
