Table 1.
Antibodies used for flow cytometry, western blot analyses, and immunofluorescent staining.
| Flow cytometry | ||||
|---|---|---|---|---|
| Target | Company | Catalog number | Clone | Fluorophore |
| Syndecan‐2 | MACS | 130‐107‐480 | REA468 | PE |
| CD90 | Biolegend | 328115 | 5E10 | AF647 |
| CD73 | Biolegend | 344015 | AD2 | FITC |
| CD105 | Biolegend | 323207 | 43A3 | APC |
| HLA DR | Biolegend | 307609 | L243 | APC |
| Ly6G | Biolegend | 127613 | 1A8 | APC |
| Ly6G | Biolegend | 127606 | 1A8 | FITC |
| CD11b | BD bioscience | 557397 | M1/70 | PE |
| F4/80 | Biolegend | 123116 | BM8 | APC |
| Western blot analyses | ||||
|---|---|---|---|---|
| Target | Company | Catalog number | Isotype | Host species |
| Syndecan‐2 | Abcam | ab191062 | IgG | Rabbit |
| ALIX | Abcam | ab117600 | IgG1 | Mouse |
| Tsg101 | Abcam | ab30871 | IgG | Rabbit |
| Syntenin‐1 | Santa Cruz | sc‐515538 | IgG2a | Mouse |
| CD63 | Santa Cruz | sc‐5275 | IgG1 | Mouse |
| CD81 | Santa Cruz | sc‐166029 | IgG2b | Mouse |
| CD9 | Santa Cruz | sc‐13118 | IgG1 | Mouse |
| β‐actin | Santa Cruz | sc‐47778 | IgG1 | Mouse |
| Histone H3 | Abcam | ab1791 | IgG | Rabbit |
| Immunofluorescent staining | ||||
|---|---|---|---|---|
| Target | Company | Catalog number | Isotype | Host species |
| Ly6G | Biolegend | 127602 | IgG2a, κ | Rat |
| CD68 | Abcam | ab125212 | IgG | Rabbit |