Fig. 5.

Relative mRNA levels for multiple genes involved in the regulation of cholesterol homeostasis (a), or that serve as markers of inflammation (b) in the brains of 141-day old Lal^+/+ and Lal^−/− mice. The details of these analyses are given in Materials and Methods. For each genotype brains were obtained from 2 male and 2 female mice, all of the FVB/N strain. The expression level for each gene in the Lal^+/+ mice was arbitrarily set at 1.0 (dashed line) so bars reflect fold-change observed in the Lal^−/− mice. Values represent the mean ± SEM. *Significantly different from the value for the Lal^+/+ controls (p<0.05). The full name of each gene in the order as it appears in the figure is: Lipa, gene that encodes LAL; Npc2, Niemann-Pick type C2; Npc1, Niemann-Pick type C1; Srebp2, sterol regulatory element-binding protein 2; Hmgcs, 3-hydroxy-3-methylglutaryl coenzyme A synthase; Hmgcr, 3-hydroxy-3-methylglutaryl-coenzyme A reductase; Cyp46a1, cholesterol 24-hydroxylase; Apoe, apolipoprotein E; Ldlr, low-density lipoprotein receptor; Soat1, sterol O-acyltransferase 1 (previously called Acat1); Lcat, lecithin:cholesterol acyltransferase; Abca1, ATP-binding cassette transporter A1; Abca2, ATP-binding cassette transporter A2; Abcg1, ATP-binding cassette transporter G1; Ccl3, chemokine (C-C motif) ligand 3 (also known as Mip1α); TNFα, tumor necrosis factor alpha; ltgax, integrin subunit alpha X (also known as CD11c); Cd14 (Cluster of differentiation 14 antigen).