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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Mol Biotechnol. 2021 Sep 22;64(2):156–170. doi: 10.1007/s12033-021-00403-x

Fig. 2.

Fig. 2

Localization in culture. A Western blot using an anti-BMP-4 GF antibody. B Western blot using an antibody that detects the His8 tag N-terminal to the pro-domain. The left panel shows the total sample (CM and cells). The second panel shows cleared CM. The third panel shows the cell pellet. The right panel shows the supernatant of the cell pellet washed with TBS. B4/4, B2/4, and MB4 were expressed ± furin as indicated. The following species are detected: BMP-4 GF (GF) at 10–15 kDa, unprocessed ligand (UP) at 55 kDa, and unprocessed MBP fusion (MUP) at 90 kDa. All samples were reduced