. Author manuscript; available in PMC: 2022 Feb 1.

Published in final edited form as: Mol Biotechnol. 2021 Sep 22;64(2):156–170. doi: 10.1007/s12033-021-00403-x

Table 1.

Estimated yields of purified BMP-4

Construct	Furin	Fraction	Purified moiety	Final purification	Yield (per L)^a
B4/4	+	CM	GF	RPC	< 20 μg
B4/4	−	CM	GF	RPC	< 20 μg
B2/4	+	CM	GF	RPC	< 20 μg
B2/4	−	CM	GF	RPC	~ 100 μg
B4/4	+	CPF	GF	RPC	~ 650 μg
B4/4	−	CPF	GF	RPC	~ 650 μg
B2/4	+	CPF	GF	RPC	~1000 μg
B2/4	−	CPF	GF	RPC	~1900 μg
MB4	+	CM	Degraded	N.A	N.A
MB4	−	CM	FL	Q + SP	~ 400 μg

300 mL cultures seeded at 3 × 10⁵ cells/mL were harvested after 10 days of growth. Volumes were matched throughout purification. Yields were estimated by BCA