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The effect of imipramine on IL-6 secretion and c-FOS expression by macrophages stimulated with LPS and/or PA. RAW264.7 macrophages were treated with 1 ng/ml of LPS, 100 μM of PA or both LPS and PA. The levels of IL-6 in culture medium after 12 and 24 h incubation (A), and c-FOS mRNA at 12 h (B) were quantified using ELISA and real-time PCR, respectively. The data are mean ± SD of one of three experiments with similar results.
