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Phosphofructokinase has little effect on hexosamine biosynthetic pathway activity in the heart. (A) Schematic of hexosamine biosynthetic pathway. (B) Pool totals and (C) isotopologue abundances of hexosamine biosynthetic pathway intermediates in hearts of GlycoLo, GlycoHi, and WT hearts. *p < 0.05, **p < 0.01, ****p < 0.0001, (panel B) one-way ANOVA or (panel C) two-way ANOVA with Tukey’s multiple comparison test, n = 4–5 hearts per group.
