Reagent	Source	Catalog
Rabbit polyclonal Anti-PAICS antibody	Millipore Sigma	AV46049 (1:1000 dilution)
HRP-linked Anti-Rabbit antibody	Cell Signaling Technologies	#7074 (1:1000 dilution)
Basal diet base	Envigo	TD.150344.PWD
12C6-glucose	Sigma Aldrich	#G7528
13C6-glucose	Sigma Aldrich	#389374
Acetonitrile	ThermoFisher	A956-1
Chloroform	Sigma Aldrich	650498
MOPS	Fisher	BP308
Mannitol	VWR	BDH9248
Sucrose	VWR	BDH9308
EGTA	Sigma Aldrich	E4378
Digitonin	Sigma Aldrich	300410
Coomassie Blue G250	Fisher	BP100
Polyacrylamide (acrylamide:bis-acrylamide 37.5:1)	BioRad	#1610158
Tricine	Sigma Aldrich	T0377
Bis-Tris	VWR	#0715
Trypsin	Promega	V511A
DTT	Sigma Aldrich	D0632
TEA-BC	Sigma Aldrich	T7408
Iodoacetamide	Sigma Aldrich	I1149
Formic Acid	Fisher	A117-50
Acetonitrile	Fisher	A955-4
LC-MS grade Water	Fisher	W6-4
Free Fatty-Acid Quantitation Kit	Sigma Aldrich	MAK044-1KT
Rat/Mouse Insulin ELISA	Millipore Sigma	EZRMMI-13 K
0.45 μm Regenerated Cellulose Syringe Filter	Fisher	F2504-7
Equipment	Source	Catalog
Orbitrap Fusion Tribrid mass Tribrid mass spectrometer	ThermoFisher	https://www.thermofisher.com/order/catalog/product/IQLAAEGAAPFADBMBCX#/IQLAAEGAAPFADBMBCX
Dionex ICS 5000	Dionex	https://www.thermofisher.com/us/en/home/industrial/chromatography/ion-chromatography-ic/ion-chromatography-systems.html
Orbitrap Elite - ETD mass spectrometer	ThermoFisher	https://assets.thermofisher.com/TFS-Assets/CMD/brochures/BR-30232-LC-MS-Orbitrap-Elite-BR30232-EN.pdf
Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer	ThermoFisher	https://assets.thermofisher.com/TFS-Assets/CMD/Specification-Sheets/PS-64048-LC-MS-Q-Exactive-HF-Orbitrap-PS64048-EN.pdf
MestReNova v.12.0.0	Mestrelab Research S.L.	https://mestrelab.com/download/mnova/
Agilent Varian DD2 spectrometer	Agilent	https://www.agilent.com/cs/library/slidepresentation/public/2.DD2console.pdf
Software	Source	Link
MetaboAnalyst 5.0	MetaboAnalyst	https://www.metaboanalyst.ca/
Prism 9.0	GraphPad	https://www.graphpad.com/scientific-software/prism/
Proteome Discoverer v1.4.1.114	ThermoFisher	https://www.thermofisher.com
Xcalibur v2.2	ThermoFisher	https://www.thermofisher.com
TraceFinder 3.3	ThermoFisher	https://assets.thermofisher.com/TFS-Assets/CMD/manuals/Man-XCALI-97728-TraceFinder-33-User-Quan-ManXCALI97728-A-EN.pdf