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. 2022 Jan 5;8:809689. doi: 10.3389/fcvm.2021.809689

Figure 5.

Figure 5

AKT inhibitor (AKTi) abolished the antioxidant and antiapoptotic effect of rutaecarpine in DOX-treated hearts. Mice were intraperitoneally injected with AKTi (20 mg/kg) every other day for 4 weeks before DOX and Rut administration. (A) ROS generation in the heart was detected by DHE staining, and the quantification of fluorescence intensity was shown right (n = 5). Scale bar 100 μm. (B) Representative TUNEL staining in the heart tissues and the TUNEL-positive nuclei were quantified (n = 3, right). Scale bar 50 μm. (C–E) Relative SOD activity, GSH content, and MDA activity in the heart; data in other groups were normalized to the vehicle group (n = 5). (F) Western blot analysis of p-AKT, Nrf-2, GCLM, HO-1, cleaved caspase-3, Bax, and Bcl-2 in the heart, and the relevant quantification was shown right (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. vehicle; #p < 0.05, ##p < 0.01 vs. DOX group; &p < 0.05 vs. DOX plus Rut group.