TABLE 3.
Reactivities of the truncated NP fragments of EBO to EBO antibody-positive samples determined by Western blotting and ELISA
| Sample | Reactivitya/antigenic index of:
|
||||||
|---|---|---|---|---|---|---|---|
| His-EBO-NP | GST-EBO-
|
GST | |||||
| NP/C-half | NP5 | NP6 | NP7 | NP8 | |||
| E1 | + | +/16 | +/8 | −/4 | −/2 | +/8 | −/1 |
| E2 | + | +/8 | −/1 | −/1 | +/4 | +/8 | −/1 |
| E3 | + | +/4 | −/1 | +/4 | −/1 | +/8 | −/1 |
| E4 | + | +/8 | −/1 | +/8 | −/2 | +/16 | −/1 |
| E5 | + | +/64 | +/64 | +/32 | −/4 | +/32 | −/1 |
| E6 | + | +/64 | +/8 | −/1 | −/8 | +/64 | −/1 |
| E7 | + | +/NTb | −/NT | +/NT | −/NT | +/NT | −/NT |
| E8 | + | +/>128 | +/32 | +/32 | +/32 | +/>128 | −/1 |
| E9 | + | +/8 | +/1 | +/2 | −/1 | −/16 | −/1 |
| E10 | + | +/>128 | +/8 | +/16 | −/8 | +/>128 | −/1 |
| E11 | + | +/>128 | +/4 | +/16 | +/8 | +/>128 | −/1 |
| E12 | + | +/>64 | +/2 | +/2 | −/2 | +/64 | −/1 |
| E13 | + | +/>128 | +/16 | +/16 | −/16 | +/>128 | −/1 |
| E14 | + | +/>128 | +/2 | +/8 | −/16 | +/>128 | −/1 |
| E15 | + | +/32 | −/4 | +/4 | +/4 | +/64 | −/1 |
| E16 | + | +/16 | +/2 | +/2 | −/2 | +/16 | −/1 |
| E17 | + | +/8 | +/2 | +/1 | −/1 | +/2 | −/1 |
| E18 | + | +/8 | +/8 | −/1 | −/2 | +/8 | −/1 |
| E19 | + | +/32 | +/2 | +/16 | +/1 | +/2 | +/1 |
| E20 | + | +/32 | +/4 | +/1 | −/1 | +/8 | −/1 |
| E21 | + | +/>128 | +/8 | +/16 | −/8 | +/>128 | −/1 |
| E22 | + | +/>128 | +/2 | −/2 | −/1 | +/64 | −/1 |
| E23 | + | +/32 | −/4 | −/2 | −/2 | +/16 | −/1 |
| E24 | + | +/>128 | +/>128 | +/>128 | +/64 | +/>128 | −/1 |
| E25 | + | +/>128 | +/32 | +/64 | −/16 | +/>128 | −/1 |
| E26 | + | +/>32 | +/>32 | +/16 | +/>32 | +/>32 | −/1 |
| No. of WBc positives | 26 | 26 | 20 | 20 | 7 | 25 | 1 |
| Avg log2 antigenic indexd | 5.2 | 2.4 | 2.6 | 2.1 | 4.9 | 0 | |
a
By Western blotting.
b
NT, not tested.
c
WB, Western blotting.
d
In the calculation of log2 antigenic indices of >32, >64, and >128, the values of the antigenic indices were regarded as 32, 64, and 128, respectively.