TABLE 4.
Reactivities of recombinant MBG NP fragments to MBG antibody-positive samples determined by Western blotting and ELISA
| Sample | Reactivitya/antigenic index of:
|
||||||
|---|---|---|---|---|---|---|---|
| His-MBG-NP | GST-MBG-
|
GST | |||||
| NP/C-half | NP5 | NP6 | NP7 | NP8 | |||
| M1 | + | +/>64 | +/2 | −/8 | −/8 | +/>64 | −/1 |
| M2 | + | +/>64 | −/2 | +/8 | −/2 | +/>64 | −/1 |
| M3 | + | +/NTb | +/NT | +/NT | −/NT | +/NT | −/1 |
| M4 | + | +/>16 | −/2 | −/2 | −/2 | +/>16 | −/1 |
| M5 | + | +/16 | +/4 | +/8 | −/2 | +/8 | −/1 |
| M6 | + | +/>128 | +/64 | +/>128 | +/64 | +/>128 | −/1 |
| M7 | + | +/16 | +/2 | −/2 | −/1 | +/8 | −/1 |
| M8 | + | +/>128 | +/4 | +/32 | −/1 | +/>128 | −/1 |
| No. of WBc positives | 8 | 8 | 6 | 5 | 1 | 8 | 0 |
| Average log2 antigenic indexd | 5.4 | 2.0 | 3.3 | 1.7 | 5.1 | 0 | |
a
By Western blotting.
b
NT, not tested.
c
WB, Western blotting.
d
In the calculation of log2 antigenic indices of >16, >64, and >128, the values of the antigenic indices were regarded as 16, 64, and 128, respectively.