TABLE 1.
Predicted PETase | GenBank entry/MGY identifier | Phylogenetic Affiliation | aa/MW (kDa) | Derived from | Expression level/solubility | Active on |
||||||
pNP-C6/-C10 | TBT | Impranil® DLN |
PCL | BHET | PET-foil | PET particles | ||||||
PET27 | WP_111881932 | Aequorivita sp. CIP111184 | 364/37.8 | Antarctic source (Li et al., 2017) | High/majority in inclusion bodies | + | + | + | + | + | + | + |
PET28 | WP_073216622 | Aequorivita viscosa | 365/38.3 | Seaweed (Li et al., 2017) | High/majority in inclusion bodies | + | + | + | + | + | - | - |
PET29 | WP_052671284 | Aequorivita vladivostokensis | 365/39.3 | Troitsa bay, Sea of Japan (Li et al., 2017) | High/majority in inclusion bodies | + | + | + | + | + | - | - |
PET30 | WP_039353427 | Kaistella jeonii | 366/37.4 | Antarctic moss (Li et al., 2017) | High/majority soluble | + | + | + | + | + | + | + |
PET38 | WP_083800582.1/GCA_ 000194605.1 | Fluviicola taffensis | 447/40.4 | River, United Kingdom (Woyke et al., 2011) | Low | - | - | N.D. | - | - | - | - |
PET53 | k99_709705_13 | Aequorivita sp. | 294/37.8 | Marine aquaculture fish tank metagenome/unpublished data University of Hamburg | Low | - | - | N.D. | - | N.D. | N.D. | N.D. |
PET57 | GUT_GENOME137663_00143 | Porphyromonas sp. | 323/36.3 | Human gut (Mitchell et al., 2019; Almeida et al., 2021) | High/majority soluble | - | - | + | + | N.D. | N.D. | N.D. |
PET58 | GUT_GENOME065712_01381 | Porphyromonas bennonis | 338/37.6 | Human gut (Mitchell et al., 2019; Almeida et al., 2021) | High/majority in inclusion bodies | - | - | - | + | N.D. | N.D. | N.D. |
PET59 | GUT_GENOME243617_00165 | Porphyromonas sp. | 345/38.4 | Human gut (Mitchell et al., 2019; Almeida et al., 2021) | High/majority soluble | - | - | + | N.D. | N.D. | N.D. |
TBT, tributyrin; BHET, bis-(2-hydroxyethyl) terephthalate; PCL, polycaprolactonate; pNP-C6/C10, para-nitrophenyl esters with chain length C6 or C10; aa, amino acids; MW, molecular weight. N.D. not determined. +, active; -, not active.
PET57-59 were extracted from the gut genomes available at: https://www.ebi.ac.uk/metagenomics/genomes/MGYG-HGUT-01059 (PET57); https://www.ebi.ac.uk/metagenomics/genomes/MGYG-HGUT-01060 (PET58) and https://www.ebi.ac.uk/metagenomics/genomes/MGYG-HGUT-00764 (PET59).