Figure 4.

Serum factors contribute to BCG-induced control of mycobacterial growth in vitro. Samples were used from n=9 healthy UK adults enrolled into human Study 1 (A) and n=9 healthy Nepalese military recruits enrolled into human Study 2 (B), all of whom received ID BCG vaccination. The direct MGIA was performed using PBMC collected at baseline and 84 days (A) or 70 days (B) post-BCG vaccination, co-cultured with BCG and either autologous serum matched to time-point, or autologous serum switched by time-point ie. post-vaccination serum cultured with baseline PBMC and baseline serum cultured with post-vaccination PBMC. Normalized BCG growth = (log₁₀ CFU of sample - log₁₀ CFU of growth control). Points represent the mean of duplicate values, boxes indicate the median value with the interquartile range (IQR) and the upper whisker extends to the largest value no further than 1.5 * IQR from the hinge, the lower whisker extends from the hinge to the smallest value at most 1.5 * IQR from the hinge. Outliers are plotted individually. A paired t-test was used to compare between time-points.