Figure 6.

Enhanced macrophage phagocytosis of BCG-GFP with sera or purified IgG following BCG vaccination. Samples were used from n=9 healthy UK adults enrolled into human Study 1. Antibody-dependent cellular phagocytosis was measured using human THP-1 derived macrophages and serum (A) or purified IgG (B) from baseline and 84 days post-BCG vaccination and the percentage of BCG-GFP that was phagocytosed was determined by flow cytometry. Points represent the mean of duplicate values, boxes indicate the median value with the interquartile range (IQR) and the upper whisker extends to the largest value no further than 1.5 * IQR from the hinge, the lower whisker extends from the hinge to the smallest value at most 1.5 * IQR from the hinge. Outliers are plotted individually. A Wilcoxon matched-pairs test was used to compare between time-points. Flow plots are shown from a single replicate of one subject at baseline and 84 days post-BCG vaccination using purified IgG (C).