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. 2022 Jan 5;14:811171. doi: 10.3389/fnmol.2021.811171

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Copyright © 2022 Bakaeva, Lizunova, Tarzhanov, Boyarkin, Petrichuk, Pinelis, Fisenko, Tuzikov, Sharipov and Surin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Light scattering (left column of panels) and fluorescent signals (right panel) of lymphocyte suspension (A) without (w/o) the addition of fluorescently labeled LPS analog (BDP-LPS) and after (B) one and (C) 3 h of incubation with BDP-LPS (10 μg/ml). The scattering of laser light (488 nm) in the direction of the beam propagation (FSC-H) and in the perpendicular direction (SSC-H) was recorded for cell selection. Events that had light scattering parameters typical for lymphocytes (gate P1 area) were accumulated (15,000 events) and graphs of the distribution of fluorescent cells were plotted (D) (fluorescence was monitored at 535 ± 15 nm).